FITC dextran is a green fluorescent dextran conjugate with excitation/emission wavelength around 495 nm/515 nm. PDF INTESTINAL PERMEABILITY ASSAY FITC-DEXTRAN - Bowdish PDF Dextran Fitc Endocytosis Assay Protocol PBS can also be used regardless.. 14) For injection, dilute 10-fold in injection solution for 2-nL/embryo injections. Molecular Probes dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor dyes (Molecular Probes dextran conjugates—Table 14.4).In particular, we would like to highlight the dextran conjugates of Alexa Fluor 488, Oregon Green and Rhodamine Green dyes, which are significantly brighter and more photostable than . FITC- dextrans represent a valuable tool to . The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group. In Vitro Vascular Permeability Assay (24-Well) - EMD Millipore PDF Dextran Conjugates - Thermo Fisher Scientific DEAE-dextran is a cationic derivative of dextran. Remove the tube from the magnet. Administration of FITC-dextran as carbopol microspheres produced a significantly greater bioavailability (33%) than after administration as chitosan (13% . It's available with the following CF® Dyes: CF®350, CF®488A, CF®633, CF®680, CF®750 and CF®770. PDF Synthesis of Caged Fluorescein Dextran Dextran is a complex branched glucan (polysaccharide derived from the condensation of glucose), originally derived from wine. Net Charge and Method of Labeling The net charge on a dextran depends on the fluorophore and the method of preparation. Turn off the pump once 12 mL of the FITC-dextran has been perfused through the rat (Figure 1D) and remove the needle from the rat's heart. The FITC-dextran is easily dissolved into PBS, could I dissolve them, and prepare 1mg/ml stocking solution, store at -25℃. FITC dextran is a green fluorescent dextran conjugate with excitation/emission wavelength around 495 nm/515 nm. Dextran 500 at Thomas Scientific Get medical attention. Inhalation: Remove exposed person to fresh air. CAS Number: 60842-46-8 Fluorescein isothiocyanate (FITC)-dextrans are synthesised by coupling fluoresceinyl isothiocyanate (5-isomer) to selected dextran fractions. Hence only one time assessment is made 3 hours after FITC-Dextran (4kDa) administration. Under healthy conditions, tight junctions in the intestinal wall will not allow the 4-6kDa FITC-d to enter the bloodstream.
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how to dissolve fitc dextran